Transcriptome of arabidopsis
Biologists have created an atlas of the expression of rhesus genes
Scientists from the A.A. Harkevich Institute of Information Transmission Problems of the Russian Academy of Sciences and Moscow State University have created a detailed atlas of gene expression for the Arabidopsis thaliana (Arabidopsis thaliana), a model object of plant genetics. This makes it possible to refine the functions of many genes and find genes previously unknown.
There are many different types of cells in a plant, all of them have the same set of genes. Cells and tissues differ because different genes work in them. Scientists have created a map, or atlas of expression, that is, the activity of the genes of the Tal rhesus, which allows you to see which genes and when they turn on in different parts of the plant. The expression map will help you learn about the work of those genes whose functions are still unknown. The rhesus has more than 70% of such genes.
The Rhesus Talia (Arabidopsis thaliana) is a small plant from the cruciferous family. It goes through the life cycle very quickly, in 1-2 months, and gives a lot of seeds. Its genome is one of the smallest among flowering plants. For these reasons, the rhesus is suitable for genetic research of plants, as, for example, a mouse is suitable for animal research. The data obtained at this facility is a starting point for experiments on other plants, including many agricultural ones.
According to scientists, they learned how genes are expressed in various organs and at different stages of plant development: from germination to flowering and seed maturation. It was possible to find genes specific to certain stages, and those that do not change activity during the life of the plant. In addition, the authors found several hundred new genes, despite the fact that the genome of the rhesus is, if not the smallest, then the most studied of all flowering plants.
The scientists collected the results of their work into a publicly available database, which they called TraVA (Transcriptome Variation Analysis). This database shows in which tissues, organs and during which period of the plant's life each of the 25,706 genes worked. In addition, it is possible to compare the activity of genes in different organs. The authors plan to supplement the database with data on gene expression of several plant species and tools that will allow comparing gene expression between different species.
The results of the study are published in the Plant Journal (Klepikova et al., A High Resolution Map of the Arabidopsis thaliana Developmental Transcriptome Based on RNA-seq Profiling).
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09.09.2016