Trichoderma heals wounds
Wound healing gel with trichoderma metabolism products will help with injury
Dmitry Yuryev, Indicator
Researchers of the Department of Biochemistry of the RUDN have developed a technology for creating a healing gel based on a substance that produces the mold fungus trichoderma. The results are published in the journal Systematic Reviews in Pharmacy (Smirnova et al., New Trichoderma harzianum Rifai F-180 - L-lysine--oxidase antitumor enzyme producer - culture liquid-based substance biotechnology).
The main treatment for skin injuries is the acceleration of cell regeneration with the help of various ointments. At the same time, the active substances of the preparations can be, among other things, the products of the vital activity of microorganisms. Fungi, bacteria and other organisms produce "ready-made" antibiotics, enzymes, growth stimulants, vitamins and amino acids, so researchers are looking for ways to turn waste products into medicines. For example, the product of vital activity from the genus of trichoderma is the enzyme L-lysine oxidase. Researchers have developed a technology that allows you to get a gel for wound healing with this enzyme. This technology does not require expensive purification of the original biological fluids and is therefore economically advantageous.
"Modern microbiology focuses on physiologically active products of the vital activity of microorganisms. The genus of fungi Trichoderma is best studied. The strain of Trichoderma harzianum Rifai F-180 is capable of producing large quantities of the protein L-lysine oxidase, which has proven antiviral and antitumor properties.
All this opens up prospects for the development of new dosage forms based on L-lysine oxidase for use in infections and skin injuries," says Irina Smirnova, Doctor of Biological Sciences, Professor of the Department of Biochemistry. academician T.T. Berezov RUDN.
The optimal concentration of the active component (one percent) was chosen by scientists based on the data obtained in previous studies. When choosing the auxiliary components of the gel, the researchers were guided by the rules – the gel base should be combined with the active component, not cause irritation, promote maximum activity of L-lysine oxidase, ensure ease of application and storage. As a result, three possible gel bases – methylcellulose, carbopol and mARS polymers – were selected and tested experimentally.
For the experiment, the researchers used nine adult guinea pigs. Mechanical damage was inflicted on their skin, and after 18 hours treatment began. The animals were divided into groups of three and gel of one of three types was applied to the wound every day for two weeks. The methylcellulose–based gel showed the worst result - wounds healed after an average of 9-10 days of treatment. The best results were shown by the use of carbopol–based gel - in pigs from this group, the wound healed after 6-7 days.
"The results of the experiment showed a high wound healing activity of 1% gel based on carbopol and culture fluid of the trichoderma fungus. At the next stage, you can proceed to preclinical trials of the drug. The proposed dosage form can be used in veterinary medicine – as a wound healing agent, for skin viral infections and ophthalmic herpes – as well as in medical cosmetology, gynecology, and skin cancer," says Irina Smirnova.
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