In search of cells for IPSC
Step by step to the medicine of the future
* – Stem cells can be obtained in other ways: an embryo can share them without any damage to development ("Sparing stem cells" [2]) or... its habitat ("Endometrium as an alternative source of stem cells" [3]). – Ed.** – The article "Organs from the laboratory" tells about the situation in the field of creating artificial organs and technologies for their production [4]. – Ed.
* – Currently, when developing gene therapy approaches, special hopes are pinned on ZFN, TALEN and CRISPR/Cas9 editing systems based on the site-specific action of nucleases in vivo. These systems usually consist of two modules, one of which recognizes the desired oligonucleotide sequence, and the other cuts the DNA chains: "Should we take a swing at... genome change?" [5], "CRISPR systems: immunization of prokaryotes" [6]. And some of the approaches are already quite "tangible", since the voices of bioethicists sound louder: "Mutagenic chain reaction: genome editing on the verge of fiction" [7]. – Ed.
* – Gene and cell engineers in general are not indifferent to blood diseases, and their efforts bring more and more tangible results – both in efficiency and safety: "Summary from the gene therapy fronts. A new strategy for neutralization of hemophilia" [12]. – Ed.
* – A rare person has not heard about the bad behavior of others and even their own for some reason poorly controlled stem cells: "Stem and branches, stem cells" [1]. The methodology for obtaining iPSCs further increases the degree of oncological alertness: the problem is the incorporation into the genome of not only vector DNA with active promoters, but also proto–oncogenes - yes, the genes of two of the four "magic" TF (c-Myc and Klf4) belong to proto-oncogenes. Therefore, they are trying in every way to find a balance between the effectiveness of reprogramming and safety, changing the spectrum of TF or completely excluding the introduction of genetically engineered structures - there is hope that in the future it will be quite easy to "pohimichit": "A snowball of problems with pluripotence" [10], "There was a simple cell, it became a stem cell" [9]. – Ed.
- biomolecule: "Sparing stem cells";
- biomolecule: "Endometrium as an alternative source of stem cells";
- biomolecule: "Organs from the laboratory";
- Biomolecule: "And whether we should take a swing at...
- genome change?";Biomolecule: "CRISPR systems: immunization of prokaryotes";
- Biomolecule: "Mutagenic chain reaction: genome editing on the verge of fiction";
- Biomolecule: "Nobel Prize in Physiology or Medicine (2012): induced stem cells";
- biomolecule: "There was a simple cell, it became a stem cell";
- biomolecule: "A snowball of problems with pluripotence";
- Sebastiano V., Maeder M.L., Angstman J.F., Haddad B., Khayter C., Yeo D.T. et al. (2011).
- In situ genetic correction of the sickle cell anemia mutation in human induced pluripotent stem cells using engineered zinc finger nucleases. Stem Cells. 29, 1717–1726;biomolecule: "Summary from the gene therapy fronts.
- A new strategy for neutralizing hemophilia";Kim J.B., Zaehres H., Araúzo-Bravo M.J., Schöler H.R. (2009).
- Generation of induced pluripotent stem cells from neural stem cells. Nat. Protoc. 4, 1464–1470;biomolecule: "How to make a neuron from fibroblast?";
- Tsai S.-Y., Bouwman B.A., Ang Y.-S., Kim S.J., Lee D.-F., Lemischka I.R., Rendl M. (2011).
- Single transcription factor reprogramming of hair follicle dermal papilla cells to induced pluripotent stem cells. Stem Cells. 29, 964–971;Alekseeva E.I. Determination of the minimum set of transcription factors sufficient for induction of pluripotency of human dermal papilla cells: thesis.
- – Moscow, 2015 – 56 p.Portal "Eternal youth" http://vechnayamolodost.ru