Reprogrammed cells: a record of productivity and precocity
Reprogramming cells will become 200 times more efficient
Anna Chvyreva, Eternal MindFate Therapeutics has introduced a method of reprogramming cells using a combination of synthetic drugs, which significantly reduces the time and increases the effectiveness of reprogramming.
Dr. Sheng Ding and his colleagues from the Scripps Research Institute in La Jolla have developed a new method for creating induced pluripotent human stem cells (iPSCs) using a combination of synthetic drugs. The new method provides a more than two-hundredfold increase in reprogramming efficiency and a reduction in the period to two weeks compared to the "old" methods using four transcription factors (Oct 3/4, Sox2, Klf4 and c-Myc).
Unlike most recent studies in this area, this method is not based on genetic modifications, which makes it safer. Dr. Ding has developed a combined chemical approach that allows the creation of iPSCs from human fibroblasts in two weeks of retroviral transduction. The iPSCs cells created by Ding's team using a three-component chemical composition can be as close as possible to human embryonic cells in terms of morphology and the degree of expression of pluripotent markers, and can also be transformed into any of the three germ sheets, both in vitro and in vivo.
Earlier this year, Dr. Ding and his team pioneered a method for creating iPSCs using proteins encoded by reprogramming genes (piPSC). The technology presented by Ding's group in the journal Nature Methods (A chemical platform for improved induction of human iPSCs) is a platform for obtaining high-quality pipscs on a commercial scale.
Scientists believe that the new method will make possible the widespread use of cell reprogramming technology for the development of new drugs and the treatment of various diseases.
Source: Fate Therapeutics: Development of Two Week Method for Generating Human Induced Pluripotent Stem Cells with 200-Fold Increase in Yield.
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27.10.2009